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Technology Platforms+ ABI 3730xl
+ Roche 454 GS-FLX System
+ Illumina Solexa 1G Genome Analyzer
Services+ Whole genome shotgun sequencing
+ BAC end sequencing
+ SNP discovery and resequencing
+ Large-scale EST sequencing
+ Primer walking
+ SAGE sequencing
+ Sequencing expertise
Creative Genomics provides sequencing service for long DNA fragments in large plasmids such as BAC, Fosmid and Cosmid, as well as whole genome shotgun sequencing. In each large-scale sequencing project, a project manager oversees every step and informs clients about the progress in a timely manner.
- Shotgun library construction
- Library quality control by sequencing 96 clones
- Clone picking and arraying into 96- or 384-well plate
- Plasmid DNA preparation
- Large-scale sequencing by ABI 3730xl
- Contig assembly
- Gap closure by primer walking
- Genome coverage >99.9%
- Template DNA and bioinformatics annotation available
Assembly:An effective collaboration between bioinformatics and nucleotide departments is essential for successfully reconstructing the original sequence from the reads. In addition to the semi-automated assembly utilizing programs such as Phred, Phrap and Consed, manual curation is applied by senior bioinformaticians to eliminate potential mis-assemblies. Supplementary strategies include primer walking and/or direct PCR sequencing. Paired end DNA sequence information is used to resolve repeat sequences and maximize the level of contig alignment. Furthermore, our in-house annotation pipeline is able to extract and display valuable information from the sequences.
Deliverables:Final report includes raw sequence texts, trace data and consensus sequence files.